Recombinant Rat DDR2 Kinase/CD167b Protein (Fc Tag)(Active)

Name: Recombinant Rat DDR2 Kinase/CD167b Protein (Fc Tag)(Active)
SKU: EWV8411673969
Price: 98.99 USD
Availability: InStock

$98.99 ~~$922.95~~

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Category : Life Science Products

Recombinant Rat DDR2 Kinase/CD167b Protein (Fc Tag)(Active)

SKU #	PKSR030218
Expression Host	HEK293 Cells

Description

Synonyms	DDR2
Species	Rat
Expression Host	HEK293 Cells
Sequence	Met1-Arg399
Accession	B1WC09
Calculated Molecular Weight	69.5 kDa
Observed Molecular Weight	110 kDa
Tag	C-hFc
Bio-activity	Measured by its ability to bind with Rat tail Collagen I in a functional ELISA.

Properties

Purity	> 80 % as determined by reducing SDS-PAGE.
Endotoxin	< 1.0 EU per g of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80. Reconstituted protein solution can be stored at 4-8 for 2-7 days. Aliquots of reconstituted samples are stable at < -20 for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.